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Test Code AFBC Culture, Acid Fast for Mycobacterium (with Smear)

Infectious

Specimen Required

Specimen Type: Varies; a detailed description of the anatomic source is required

Sputum samples: it is recommended that three samples be collected at 8-24 hour intervals (24 hours is best) and should include at least one first morning specimen.

Urine samples: a first morning specimen must be processed without delay. 24 hour collections are not acceptable.

 

Container / Tube:

*  Sterile Container (body fluids, respiratory, tissue, urine)

*  BLUE Amies Culturette swab (wound)

*  YELLOW top SPS Isolator Microbial Tube (bone marrow - available from the lab)

CPT Codes

87015: Mycobacterial Culture, Concentration

87206: Mycobacterial Smear

87016: Mycobacterial Culture

Special Instructions

For optimal recovery, fresh tissue or body fluid is preferred. If unable to attain ample specimen in a sterile container, a blue Culturette swab may be used.

 

If the specimen container is a Culturette swab and multiple tests are requested, collect a second swab to maximize test sensitivity.

 

If the specimen cannot be delivered to the laboratory within 1 hour it should be refrigerated to decrease overgrowth with contaminating organisms.

Testing Schedule

Days Performed: Monday through Sunday

 

Times Performed:

Smears: Day Shift only (0600 - 1430)

Cultures: Broth is read continuously via instrumentation & culture slants are read once per week 

 

Turn-around Time:

Smears: Resulted within 24 hours

Cultures: Positive cultures are reported as soon as an organism is detected; negative cultures will be final at 6 weeks (preliminary reports are autofiled at 7 days then weekly thereafter)